Title:Evaluation of deoxyribonuclease 1-Like 3 as a potential regulator for immune activation in juvenile-onset systemic lupus erythematosus patients: a case-control study

Author:Eman Eissa, Basma M Medhat, Botros Morcos, Dalia Dorgham, Rania Kandil, Nehal El-Ghobashy and Naglaa M. Kholoussi

Abstract:Objectives: This study aimed to evaluate the expression pattern of Deoxyribonuclease1-Like 3 (DNase1L3), an endonuclease that catalyses the degradation of chromatin within apoptotic or necrotic cells, at the mRNA and protein levels and their association with the disease activity and inflammatory markers in Juvenile-onset systemic lupus erythematosus (jSLE) patients.
Methods: 36 Juvenile-onset systemic lupus erythematosus patients and 25 healthy controls were included in the study. DNase1L3 and Interleukin (IL)-1β expression in peripheral blood were determined using qRT-PCR, while ELISA was used to determine plasma levels of DNase1L3 and tumour necrosis factor (TNF)-α.
Results: DNase1L3 expression pattern at the mRNA and protein levels was significantly lower while IL-1β and TNF-a levels were significantly higher in Juvenile-onset systemic lupus erythematosus patients than healthy controls. There were significant negative associations between DNase1L3 expression at mRNA and protein levels with SLEDAI-2K of patients. Also, DNase1L3 plasma levels of patients were inversely correlated with IL-1β levels in a significant manner.
Conclusion: DNase1L3 could be involved in the immune regulation of jSLE patients and could be considered as a potential regulator of immune activation in those patients.
Keywords: DNase1L3, Juvenile-onset systemic lupus erythematosus (jSLE), inflammatory cytokines, IL-1b, TNF-α.
«Back         Download this article as PDF
✖