Title:Effect of obesity on serum IL10 concentrations and messenger RNA expression in women with metabolic syndrome

Author:Moushira Zaki, Hala T El-Bassyouni, Eman R Youness, Walaa A Basha, Maha Abdelhadi Ali, Wagdy KB Khalil, Sara M Abdo and Walaa Yousef

Abstract:Objectives: In humans, the probable role of anti-inflammatory cytokines in obesity is unidentified. The objective of this work was to investigate serum IL-10 concentrations and the messenger RNA expression (mRNA) in peripheral blood of 30 obese women with metabolic syndrome (MS) and 20 lean healthy controls matching sex and age.
Methods:In this cross-sectional study, 50 Egyptian women (age < 45 years) were included. Blood pressure, anthropometric measurements, lipid parameters, obesity indices and HOMA-IR were measured. The expression of IL-10 in blood was assessed by extraction of RNA followed by real time PCR analysis. The IL-10 level in serum was assessed using enzyme linked immunosorbent assay (ELISA).
Results:Women with MS had lower values of IL-10 and significant higher levels of blood pressure, lipid parameters, obesity measures and HOMA-IR compared to control women (all P < 0.01). Similarly, mRNA expressions were down regulated in MS women compared to the control group (P = 0.003).
Conclusion: Our study demontstrated that obese women have low levels of IL-10, suggesting that this anti-inflammatory cytokine plays a crucial role in MS risk and obesity-related problems. Moreover, low mRNA expression suggests the contribution of genes to insulin-resistant states and the metabolic syndrome in obese women.
Keywords: Interleukin 10; metabolic syndrome; obesity; mRNA expression; cytokines.
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