Title:The genes expression of NF-κB inflammation pathway in treated celiac disease patients

Author:Yasaman Hajinabi, Elham Aghamohammadi Khamene, Flora Forouzesh, Fahimeh Sadat Gholam-Mostafaei, Seyed Abedin Hosseini Ahangari, Reza Mahmoudi Lamouki and Mohammad Rostami Nejad

Abstract:Objectives: Celiac disease (CD) is a heritable chronic inflammatory disease that generally leads to a wide spectrum of clinical symptoms. The present research aimed to investigate whether the expression of key genes (NF-kB, REL, and TNFAIP3) induces inflammatory meditated NF-kB signaling changes in patients treated with a gluten-free diet compared to the healthy group.
Methods: Biopsy specimens from the distal duodenum and blood sampling were collected from 50 patients with CD (37.06 ± 7.02 years old) under gluten-free diet for at least 1 year and 50 healthy individuals (34.12 ± 4.90 years old) served as a control group. RNA was extracted from samples, cDNA synthesised and primer pairs were designed for NF-kB, REL, and TNFAIP3 gene expression. Quantitative real-time PCR was used to analyze the relative gene expression.
Results: A total of 50 CD patients (72% men and 23 % women) were included in this study. The results showed that the expression of NF-ᴋB1 and c-Rel in tissue sample and blood samples did not have a significant difference compared to the control group (P > 0.05), whereas the expression of TNFAIP3 was significantly lower than the control group (P< 0.05).
Conclusions: Generally, it seems that the disrupted gene pattern of the NF-kB pathway can affect the optimal immune response control, indicating some interactive inflammatory reactions in CD patients. These results light unknowns in interactive inflammatory reactions in CD patients and explain the common complex immune reaction in CD.
Keywords: Celiac disease, gluten-free diet, NF-kB, c-Rel, TNFAIP3.
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